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Basic Info.
Model NO.
R431002/R431003
Content
Standard
Usage
Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, Isolate Mirna Small Rna Molecules
Source
for Lab Research
Habit Appellation
Hipure Universal Mirna Kit
Application
Industry, Scientific Research, Health, Environmental Protection, Agriculture, Rt-PCR, Northern Blot, Poly a+Purification
Property
Biochemical Reagent
Main Functions
Isolation Mirna and Other Small Rna Molecules 18nt
Applications1
Rt-PCR, Northern Blot, Poly a+Purification
Applications2
Ucleic Acid Protection and in Vitro Translation
Purification Method
Mini Spin Column
Purification Technology
Silica Technology
Process Method
Manual (Centrifugation or Vacuum)
Sample Type
Animal Tissues, Adherent Cells, Suspension Cells
Sample Amount1
Eukaryotic Culture Cells: ≤ 10^7
Sample Amount2
Animal Tissue:<100mg, Yeast Culture Cells:<5 X10^7
Sample Amount3
Bacteria:<10^9
Elution Volume
≥15μl
Time Per Run
≤40 Minutes
Sample
Free Sample for Test
After-Sales Service
Technical Support
Shipping
FedEx, DHL, Door to Door
Transport Package
Box
Specification
50 Preps/ 250Preps
Trademark
Magen Biotech
Origin
China
Product Description
HiPure Universal miRNA Kit
| CAT NO | PRODUCT NAME | SIZE | PRICE |
| R431002 | HiPure Universal miRNA Kit | 50 preps | $174.00 |
| R431003 | HiPure Universal miRNA Kit | 250 preps | $746.00 |
△ Introduction
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples. Alternatively, a miRNA-enriched fraction and a total RNA (>200nt) fraction can be purified separately.
This Kit combines phenol/guanidine-based lysis of samples and silica membrane-based purification of total RNA.MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of tissues, to inhibit RNases, and also to remove most of the cellular DNA and proteins from the lysate by organic extraction.
△ Details
| Features | Specifications |
| Main Functions | Isolation miRNA and other small RNA molecules(18nt), from cultured cells and various animal and human tissues, using MagZol reagent and column |
| Applications | RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Animal tissues, adherent cells, suspension cells, bacteria, etc |
| Sample amount | Eukaryotic culture cells: ≤ 10^7, Animal tissue:<100mg, Yeast culture cells:<5 x10^7, Bacteria:<10^9 |
| Elution volume | ≥15μl |
| Time per run | ≤40 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
△ Advantages
- High quality - one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Fast - several samples can be extracted in 40 minutes
- High applicability - samples including animals, plants, bacteria, cells, etc.
- High concentration - efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity
△ Kit Contents
| Contents | R431002 | R431003 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure RNA Mini Columns | 100 | 2 x 250 |
| 2ml Collection Tubes | 100 | 2 x 250 |
| MagZol Reagent | 60 ml | 270 ml |
| Buffer RWC | 20 ml | 80 ml |
| Buffer RW2* | 20 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 30 ml |
△ Storage and Stability
MagZol Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
