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Basic Info.
Model NO.
R480201 / R480202
Content
Standard
Usage
Laboratory Reagents, Analytical Reagents, Teaching Reagents, Extract Rna From Liquid Samples by Salting out
Source
Laboratory Production
Habit Appellation
Magzol Ls Reagent
Application
Industry, Scientific Research, Health, Environmental Protection, Agriculture, Rt-PCR, Northern Hybridization, Poly (a) Enrichmen
Property
Biochemical Reagent
Applications1
Extract Rna From Liquid Samples by Salting out
Applications2
Northern Blot Analysis, DOT Blot Hybridization
Applications3
Rnase Protection Assay, Molecular Cloning
Purification Technology
Acid Phenol Guanidine Isothiocyanate
Process Method
Manual (Centrifugation)
Sample Type1
Various Liquid Samples
Sample Type2
Suspended Cells, Bacteria, Trace Fungi
Sample Amount1
Flexible
Purification Method
Reagent
Elution Volume
Variation with Sample Size
Time Per Run
Variation with Sample Size
Sample
Free Sample
After-Sales Service
Technical Support
Transport Package
Box
Specification
100ml / 200ml
Trademark
Magen Biotech
Origin
China
HS Code
90279000
Production Capacity
100000 Per Month
Product Description
MagZol LS Reagent
| CAT NO | PRODUCT NAME | SIZE | PRICE |
| R480201 | MagZol LS Reagent | 100 ML | $124.00 |
| R480202 | MagZol LS Reagent | 200 ML | $224.00 |
△ Introduction
MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi.
The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
This method is suitable for small quantities of tissue (<100mg) and cells (<5 X106), and large quantities of tissue (up to 1g) and cells (<108), of human, animal, plant, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour.
Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, poly(A) + selection, in vitro translation, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.
△ Details
| Features | Specifications |
| Main Functions | Extract RNA from liquid samples by salting out method |
| Applications | RT-PCR, Northern hybridization, poly (a) enrichment, etc. |
| Purification technology | Acid phenol guanidine isothiocyanate |
| Process method | Manual (centrifugation) |
| Sample type | Various liquid samples |
| Sample amount | Flexible |
| Elution volume | Variation with sample size |
| Time per run | Variation with sample size |
△ Advantages
- Flexible - sample amount can be adjusted according to the demand
- Cost performance -the most economical nucleic acid extraction technology
△ Storage and Stability
MagZol LS Reagent should be stored at 2-8°C upon arrival and is stable for at least 24 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance.
Experimental data
